产品说明
特异性
Reacts with the Poliovirus 1, 2 and 3.
应用
IFA at 1:100-1:400.
Dilute with buffer pH 7.5-8.0 to desired working volume.
Final working dilutions must be determined by end user.
SUGGESTED IFA PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μl diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μl diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Detect Poliovirus Blend using this Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1 & 613-F1-B5-E5 validated for use in IF.
储存及稳定性
Maintain at -20°C in convenient undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
产品性质
| 质量水平 | 300 |
| 生物来源 | mouse |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | 583-G8-G2-A4, monoclonal 591-B1-H7-D1, monoclonal 613-F1-B5-E5, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunofluorescence: suitable |
| 同位素/亚型 | IgG |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
