Anti-Pink1 Antibody, clone N4/15

产品说明

一般描述

Mutations in the PTEN-induced kinase 1 (PINK1) gene located within the PARK6 locus on chromosome 1p35-p36 have recently been identified in patients with recessive early-onset Parkinson′s disease. Data suggests that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance.

特异性

This antibody recognizes the cytoplasmic domain of Pink1. Other Homologies: Mouse (81% Sequence Homology) and Rat (82% Sequence Homology)

免疫原

Recombinant protein corresponding to the cytoplasmic domain of human Pink1.
Epitope: Cytoplasmic domain

应用

Anti-Pink1 Antibody, clone N4/15 is a highly specific mouse monoclonal antibody & that targets PINK1 & has been tested in western blotting.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pink1 in 10 µg of human testis tissue lysate.

Immunohistochemistry Analysis: A representative lot detected Pink1 in rat caudate putamen tissue (Prof. J. Trimmer, University of California, Davis.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

质量

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pink1 in 10 µg of human skeletal muscle tissue lysate.

目标描述

~60 kDa observed. Uniprot describes two isoforms at 63 kDa and 30 kDa The full length protein of Pink1 is observed at 63 kDa is the full length PINK1; however, it is proteolytically cleaved to a ~52 kDa form upon entry into the mitochondria (Jin, S. M., et al. (2010). J Cell Biol. 191(5):933-942.).

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human skeletal muscle tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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