Anti-Piccolo Antibody, clone 4G3.3

产品说明

一般描述

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

特异性

This antibody recognizes piccolo.

免疫原

GST-tagged recombinant protein

应用

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Sub Category
Synapse & Synaptic Biology
This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.
Research Category
Neuroscience

质量

Routinely evaluated by western blot on rat brain lysate.

目标描述

547 kDa

外形

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.
Ammonium Sulfate Precipitation

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain lysate, mouse cerebellum

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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