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Anti-Phosphothreonine Antibody, clone RM102

品牌
Sigma-Aldrich
货号
MABS499
规格纯度
clone RM102, from rabbit, purified by affinity chromatography
参考价格
5426.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.

免疫原

Mix of recombinant protein and peptide corresponding to All Phosphothreonine.

应用

Immunocytochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on Calyculin A/Okadaic Acid treated A431 cells
Immunohistochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on human colon cancer tissue
Immunoprecipitation Analysis: A representative lot of this antibody at 1:500 dilution immunoprecipitated phosphothreonine modified proteins from Calyculin A/Okadaic Acid treated A431 cell lysate
Anti-Phosphothreonine, clone RM102 is an antibody against Phosphothreonine for use in WB, ICC, IH, IP.

质量

Evaluated by Western Blotting on Calyculin A/Okadaic Acid treated HeLa cell lysate

Western Blotting Analysis: A 1:2000 dilution of this antibody detected phosphothreonine modification on Calyculin A/Okadaic Acid treated HeLa cell lysate

目标描述

N/A kDa observed

外形

Protein A purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified antibody
antibody product typeprimary antibodies
克隆RM102, monoclonal
纯化方式affinity chromatography
species reactivityhuman
species reactivity (predicted by homology)all
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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