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Anti-phospho-FKHRL1/FOXO3A (Thr32)

品牌
Sigma-Aldrich
货号
ABS554
规格纯度
from rabbit, purified by affinity chromatography
参考价格
4177.49 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Forkhead box protein O3 (UniProt: O43524; also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1) is encoded by the FOXO3 (also known as FKHRL1, FOXO3A) gene (Gene ID: 2309) in human. Forkhead box protein O3 is a transcriptional activator that riggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Its DNA binding domain located in amino acids 157-251. The nuclear localization signal is located in amino acids 242-259. In the cytoplasm it is present in an inactive state and it translocates to the nucleus upon oxidative stress and in the absence of survival factors. In the presence of survival factors such as IGF-1, it is phosphorylated on Thr32 and Ser253 by AKT1/PKB. The phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Forkhead box protein O3 deacetylation by SIRT1 or SIRT2 stimulates its interaction SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation. A chromosomal aberration involving FOXO3 is found in secondary acute leukemias.

特异性

This rabbit polyclonal antibody detects Forkhead box protein O3 in human cells. It targets an epitope within 11 amino acids surrounding phosphorylated Thr32 from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphorylated Thr32 from the N-terminal region of human Forkhead box protein O3.

Epitope: N-terminus

应用

Anti-phospho-FKHRL1/FOXO3A (Thr32), Cat. No. ABS554, is a rabbit polyclonal antibody that detects Forkhead box protein O3 and has been tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Research Category
Signaling
Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked signal in HEK293 cells treated with IGF.

Immunohistochemistry Analysis: A 1:150 dilution from a representative lot detected phospho-FKHRL1/FOXO3A (Thr32) in human tonsil, human small intestine, and human liver cancer tissues.

质量

Evaluated by Western Blotting in HEK293 treated with IGF stimulation cell lysate.

Western Blotting Analysis: A 1:100 dilution of this antibody detected phospho-FKHRL1/FOXO3A (Thr32) in lysate from HEK293 cells treated with IGF (25 ng/mL for 10 min.).

目标描述

~75 kDa observed; 71.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
technique(s)immunohistochemistry: suitable (paraffin)
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登记号NP_001446.1
UniProt登记号O43524
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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