Anti-Peripherin Antibody, clone 8G2

产品说明

一般描述

Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig′s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

特异性

Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.

免疫原

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE

应用

Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.

Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.

Optimal working dilutions must be determined by end user.
Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.
Research Sub Category
Sensory & PNS

Neuronal & Glial Markers
Research Category
Neuroscience

目标描述

57kDa

外形

Unpurified
UnPurified mouse culture supernatant containing no preservatives.

储存及稳定性

Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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