产品说明

一般描述

PDE9A is a ubiquitous protein belonging to the cyclic nucleotide phosphodiesterase family which regulate the signaling of cyclic nucleotides such as cGMP and cAMP. PDE9A has a higher affinity for cGMP. It contains a catalytic site that associates with cofactors and hydrolyzes cGMP thereby terminating cGMP signaling for a number of cellular processes. PDE9A has been indirectly linked to signaling pathways involved in cognitive processes. Previous studies suggested that inhibition of PDE9A enhanced long-term potentiation (a widely studied mechanism of learning and memory); PDE9A may also effect changes in the plasticity of hippocampal neurons which are also important for certain types of cognitive processes. In addition to the brain, PDE9A is also highly expressed in the spleen, prostate, colon, kidney, and heart and may be involved in a range of physiological processes in these systems, via cGMP-amplified signaling.

免疫原

KLH-conjugated linear peptide corresponding to human PDE9A.

应用

Western Blot Analysis: A representative lot of this antibody detected PDE9A in human liver tissue lysate, and in mouse spleen and liver tissue lysates.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected PDE9 in human prostate tissue, and in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
Detect PDE9A using this Anti-PDE9A Antibody validated for use in Western Blotting, IHC(P).

质量

Evaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE9A in 10 µg of human spleen tissue lysate.

目标描述

~75 kDa observed. Uniprot describes 16 isoforms produced by alternative splicing, which have been observed at various molecular weights. (Kruse, L.S., et al. (2009). Brain Res. 1281: 25-34; Rentero, C., et al. (2003). Biochem Biophys Res Commun. 301(3):686-692.)

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human spleen tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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