产品说明
一般描述
Programmed cell death protein 1 (PD-1) (UniProt Q15116), also known as CD279, hPD-1, hPD-I, hSLE1, Protein PD-1, Systemic lupus erythematosus susceptibility 2) is a type I single transmembrane protein encoded by the PDCD1 gene in human. Maintenance of peripheral tolerance by preventing the development of autoimmunity due to T lymphocytes specific for self-antigens is essential for homeostasis of the immune system. PD-1 receptor CD279 and its ligands PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC; CD273) play a vital role in the induction and maintenance of peripheral tolerance. Upregulated PD-1 expression is seen on conventional CD4+ CD25- T cells (Tconv) when exposed to mesenchymal stem cells, resulting in increased Tconv apoptosis due to PD-1 stimulation by the MSC PD-L2.
免疫原
Epitope: Extracellular domain
KLH-conjugated linear peptide corresponding to the extracellular domain of human PD-1.
应用
Anti-PD-1 Antibody, clone 16A2.1 is an antibody against PD-1 for use in Western Blotting, Flow Cytometry.
Flow Cytometry Analysis: 2.0 µg of this antibody from a representative lot detected PD-1 in 1X10E6 Staph enterotoxin B treated human peripheral blood mononuclear cells.
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology
质量
Evaluated by Western Blotting in TPA/LPS treated THP-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected PD-1 in 10 µg of TPA/LPS treated THP-1 cell lysate.
目标描述
~32 kDa observed
外形
Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 16A2.1, monoclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
technique(s) | flow cytometry: suitable western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_005009 |
UniProt登记号 | Q15116 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |