Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)

产品说明

一般描述

Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).

免疫原

recombinant, human p53 expressed in E. coli
Epitope: within amino acids 37-45 of p53

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue

其他说明

Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息