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Anti-O-Linked N-Acetylglucosamine Antibody, clone RL2

品牌
Sigma-Aldrich
货号
MABS157
规格纯度
clone RL2, from mouse
参考价格
6358.33 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Posttranslational modification of proteins by β-linked N-acetylglucosamine (β-GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked β-GlcNAc or simply O-GlcNAc. O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants. Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures. In addition, O-GlcNAcylation is a highly dynamic and reversible process. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins. In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling. Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation. In Alzheimer’s diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated. Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated.

特异性

Target structure is not species-specific.
Specifically recoginzes O-linked N-Acetylglucosamine (O-GlcNAc) moieties on O-GlcNAcylated proteins and peptides. Exhibits little reactivity toward free GIcNAc and no reactivity toward GalNac. Galactosyltransferase treatment of O-GlcNAcylated proteins results in galactosylation of O-GlcNAc via β1-4 linkage and a complete loss of binding by clone RL2 (Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).

免疫原

Pore complex-lamina fraction purified from rat liver nuclear envelopes corresponding to Rat O-Linked N-Acetylglucosamine.

应用

Anti-O-Linked N-Acetylglucosamine Antibody, clone RL2 is an antibody against O-Linked N-Acetylglucosamine for use in Western Blotting, Immunocytochemistry, Affinity Binding Assay, Electron Microscopy, Immunoprecipitation.
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected O-Linked N-Acetylglucosamine in HeLa cells.
Immunocytochemistry Analysis: A representative lot immunostained nuclear envelopes, but not the nuclear interior, of digitonin-permeabilized HeLa cells. Clone RL2 stained the nuclear interior only among Triton X-100-permeabilized HeLa cells without intact nuclear envelopes (Adam, S.A., et al. (1990). J. Cell Biol. 111(3):807-816).
Affinity Binding Assay: A representative lot was radiolabeled with 125I and studied for its binding characteristics toward isolated rat liver nuclear envelopes (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-1156).
Electron Microscopy: A representative lot localized the O-GlcNAc immunoreactivity in isolated rat liver nuclear envelopes (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-1156).
Western Blotting Analysis: A representative lot detected O-GlcNAcylated proteins in rat liver nuclear envelopes preparations (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-1156; Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).
Immunoprecipitation Analysis: A representative lot immunoprecipitated O-GlcNAcylated proteins from solubilized rat liver nuclear envelopes preparations. Pretreatment of nuclear envelopes preparations with galactosyltrarnsferase prevented the immunoprecipitation of glycoproteins by clone RL2 (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-1156; Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected O-Linked N-Acetylglucosamine in 10 µg of HeLa cell lysate.

目标描述

Variable, depending on the size(s) of the O-GlcNAcylated protein(s).

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆RL2, monoclonal
species reactivity (predicted by homology)all
technique(s)affinity binding assay: suitable
electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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