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Anti-NRF-1 Antibody

品牌
Sigma-Aldrich
货号
ABE1049
规格纯度
serum, from goat
参考价格
4681.07 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), reulsting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

特异性

Expected to react with all four spliced isoforms of human NRF-1 reported by UniProt (Q16656). This antiserum bound DNA-NRF-1 complex as evidenced by a specific supershift of NRF-1, but not NRF-2, DNA complex in EMSA (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).

免疫原

Recombinant human NRF-1.

应用

Research Sub Category
Transcription Factors
Anti-NRF-1 Antibody is an antibody against NRF-1 for use in Western Blotting, Electrophoretic Mobility Shift Assay.
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing human mitochondrial transcription specificity factor genes (TFB1M & TFB2M) NRF-1-binding site and HeLa nuclear extract (NE) or purified recombinant NRF-1 (Gleyzer, N., et al. (2005). Mol. Cell. Biol. 25(4):1354-1366).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing rat cytochrome C NRF-1-binding site and HeLa nuclear extract (NE), purified HeLa NRF-1 or recombinant NRF-1. No shift by this antiserum of HeLa NE proteins complexed with oligonucleotide containing mouse COXVb gene NRF-2-binding site was observed (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: An 1:200 dilution of this antibody detected NRF1 in 10 µg of HeLa nuclear extract.

目标描述

~68 kDa observed. 53.54 kDa (isoform Long), 46.16 kDa (isoform Short), 36.05 kDa (isoform 3), 55.67 kDa (isoform 4) calculated.

外形

Goat polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源goat
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman
technique(s)electrophoretic mobility shift assay: suitable
western blot: suitable
NCBI登记号NP_001035199
UniProt登记号Q16656
运输dry ice
Gene Informationhuman ... NRF1(4899)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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