Anti-Neurophysin 1 Antibody, clone PS 38

产品说明

一般描述

Neurophysins are linear cycteine-rich proteins containing 93-95 amino acids. Two types of neurophysins, NPI and NPII are found in most mammalian species and they serve as carriers of oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone is synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or VAP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. VAP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. VAP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).

特异性

Clone PS 38 detects neurophysin 1 (oxytocin-associated NP), but not ocytocin/oxytocin or neurophysin 2 (vasopressin-associated NP)

免疫原

KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes corresponding to rat Neurophysin 1.
Epitope: Neurophysin 1

应用

This Anti-Neurophysin 1 Antibody, clone PS 38 is validated for use in Western Blotting, Immunohistochemistry, Radioimmunoassay for the detection of Neurophysin 1.
Immunohistochemistry: A representative lot detected oxytocin (OT)-synthesizing neurons in the hypothalamic supraoptic (SON) and neural lobe (NL) regions of paraffin-embedded rat hypothalamus and pituitary tissue sections (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in rat pituitary tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.

目标描述

~12 kDa observed

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息