产品说明
一般描述
Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.
特异性
Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.
免疫原
synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
应用
Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
生化/生理作用
Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
储存及稳定性
For extended storage, freeze at -20 ℃ in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | NSE-P2, monoclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~47 kDa |
species reactivity | human, rat |
浓度 | ~1 mg/mL |
technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum. western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts. |
同位素/亚型 | IgG1 |
UniProt登记号 | P09104 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... ENO2(2026) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |