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Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

品牌
Sigma-Aldrich
货号
MABN140
规格纯度
clone 27-4, from rabbit
参考价格
6358.33 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

免疫原

Recombinant protein corresponding to mouse NeuN.

应用

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex and hippocampus primary cultures.

Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected NeuN in mouse cerebellum and hippocampus tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4 detects level of NeuN (rabbit monoclonal) & has been published & validated for use in ICC, Western Blotting & IHC.

质量

Evaluated by Western Blot in mouse brain nuclear extract.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

目标描述

~46/48 kDa observed

外形

Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain nuclear extract

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆27-4, monoclonal
species reactivityrat, mouse
species reactivity (predicted by homology)human (based on 100% sequence homology)
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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