产品说明
一般描述
Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.
特异性
The antibody has been reported to block cell adhesion and herpes virus entry.
免疫原
3T3 cells expressing human Nectin-2.
应用
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- flow cytometry
生化/生理作用
Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | R2.5254.2, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~72 kDa |
| species reactivity | rat, human, monkey, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells indirect ELISA: suitable western blot: suitable |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NECTIN2(5819), PVRL2(5819), PVRL2(308417) mouse ... Nectin2(19294) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
