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Anti-NB-2/Cntn5 Antibody, clone 1A6

品牌
Sigma-Aldrich
货号
MABN877
规格纯度
clone 1A6, from rat
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Contactin-5 (UniProt P68500; also known as Neural recognition molecule NB-2) is encoded by the Cntn5 gene (Gene ID 244682) in murine species. Contactin-5 (NB-2) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein belonging to a subgroup of the immunoglobulin superfamily of proteins known as contactins. Five of the six contactins, Contactin-1, contactin-2/TAG-1, contactin-3/BIG-1, contactin-4/BIG-2, and contactin-5/NB-2 are expressed by DRG neurons. Of these, contactin-1, TAG-1, and BIG-2, are also expressed by motor neurons. NB-2 plays an important role in synapse formation in the developing auditory system in rodents. Expression of NB-2 is seen in the central auditory pathways just before the onset of hearing in rodents and is localized at glutamatergic synapses in the postnatal auditory brainstem. NB-2-knockout mice exhibit abnormal responses to auditory stimuli in both neuronal excitability and behavior, and have delayed auditory brainstem response waves. In addition, NB-2 deficiency in mice causes a significant reduction in the number of synapses and induces apoptosis in a subset of neurons in the auditory brainstem. NB-2 is produced with an N-terminal signal peptide (a.a. 1-23) and a C-terminal propeptide (a.a. 1072-1098) sequence, the removal of which yields the mature GPI-anchored glycoprotein (a.a. 24-1071) with six C2-type Ig-like domains (a.a. 98-659) and four type-III fibronectin domains (a.a. 672-1066).

特异性

Clone 1A6 detected NB-2 in the synaptosomal, but not mitochondrial, fraction from mouse cerebral cortex homogenate (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).

免疫原

Human IgG Fc-tagged recombinant mouse NB-2/Cntn5.

应用

Western Blotting Analysis: 2.0 ug/mL from a representative lot detected NB-2 in postnatal P14 and adult mouse brain tissue lysates (Courtesy of Dr. Yasushi Shimoda, PhD, Nagaoka University of Technology, Nagaoka, Japan).
Immunocytochemistry Analysis: A representative lot detected punctate NB-2 immunoreactivity on the dendrites of cultured embryonic E17 mouse hippocampal neurons. A partial overlap of NB-2 immunoreactivity with those of APLP1 and synapsin was seen (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).
Immunofluorescence Analysis: A representative lot detected NB-2 immunoreactivity among parvalbumin-/PV-positive proprioceptive sensory neurons by fluorescent immunohistochemistry staining of postnatal day 6 (p6) mouse dorsal root ganglion (DRG) cryosectoins (Ashrafi, S., et al. (2014). Neuron. 81(1):120-129).
Western Blotting Analysis: A representative lot detected NB-2 in presynaptic preparations from the spinal cords of postnatal p6 to p7 mice (Ashrafi, S., et al. (2014). Neuron. 81(1):120-129).
Western Blotting Analysis: A representative lot detected NB-2 in the synaptosomal fractions from mouse cerebral cortex homogenate (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).
Research Category
Neuroscience
This Anti-NB-2 Antibody, clone 1A6 is validated for use in Western Blotting, Immunocytochemistry, Immunofluorescence for the detection of Cntn5.
Research Sub Category
Developmental Neuroscience

质量

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2a.

目标描述

~130 kDa observed. Target band size appears larger than the calculated molecular weights of 120.7/118.0/115.1 kDa (Prepo-/Pro-/mature NB-2) due to glycosylation.

外形

Protein G purified.
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆1A6, monoclonal
species reactivitymouse
technique(s)immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001164258
UniProt登记号P68500

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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