Anti-Myb Antibody, clone 8F12.1

产品说明

一般描述

Transcriptional activator Myb (UniProt: P10242; also known as Myb, Proto-oncogene c-Myb) is encoded by the MYB gene (Gene ID: 4602) in human. Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5′-YAAC[GT]G-3′ and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. It is negatively regulated by microRNA-155. It consists of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain, and a C-terminal domain involved in transcriptional repression. MYB gene is aberrantly expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Myb protein is also reported to bind to HTLV-I long terminal repeat (LTR) sequences at four distinct regions in a sequence specific manner and activates LTR-mediated transcription. (Ref.: Dasgupta, P., et al. (1992). J. Virol. 66(1): 270-276).

特异性

Clone 8F12.1 specifically detects human Myb protein. It targets an epitope within 154 amino acids in the C-terminal region.

免疫原

GST/His-taged recombinant protein fragment corresponding to 154 amino acids from the C-terminal region of human Transcriptional activator Myb.

应用

Research Category
Epigenetics & Nuclear Function
Anti-Myb, clone 8F12.1 Antibody, Cat. No. MABE659, is a highly specific mouse monoclonal antibody that targets Myb and has been tested in Western Blotting.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myb in 10 µg of Jurkat cell lysate.

目标描述

~80 kDa observed; 72.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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