Anti-Mucin MUC5AC Antibody, clone CLH2

产品说明

一般描述

Mucins are high molecular weight glycoproteins that are heavily glycosylated with oligosaccharide side chains linked O-glycosidically to the protein backbone. At least nine mucin genes have been identified to date. The pattern of tissue and cell-specific expression of these mucin genes suggests a distinct role for each member of the diverse mucin family. In epithelial cancers, many of the phenotypic markers for pre-malignant and malignant cells have been found on the carbohydrate and peptide moieties of mucin glycoproteins. Deregulation of tissue and cell-specific expression of mucin genes has also been described in epithelial cancers. At present, the role of mucin glyoproteins in various states of epithelial cell carcinogenesis, including the pre-neoplastic state and metastasis, is under investigation.

特异性

Antibody MAB2011 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6, or 7.

Immunoreactivity of MAB2011 has been detected in the following normal human epithelial tissues: bronchus, endocervix, stomach, and gallbladder. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa. The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation.

The CLH2 antibody shows a weak reactivity with a high molecular weight smear characteristic of mucin from native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma .

The antibody was generated by immunizing mice with a synthetic tandem repeat peptide The antibody recognizes the sequence TTSTTSAP within the tandem repeat of MUC5AC (1). It further recognizes glycosylated as well as unglycosylated MUC5AC.

免疫原

Directed against a synthetic peptide of the MUC5AC tandem repeat.

应用

Anti-Mucin MUC5AC Antibody, clone CLH2 detects level of Mucin MUC5AC & has been published & validated for use in ELISA, IP, WB, IC, IH(P).
Research Sub Category
Tumor Markers
Western blot:Clone CLH2 shows a weak reactivity with a high molecular weight smear characteristic of mucin from a native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma. Typical dilution is 1:100.

Immunocytochemistry

Immunohistochemistry. Effective on frozen and paraffin sections at 1:1000-1:2000 dilution.

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer

外形

Purified immunoglobulin. Liquid at 1 mg/mL in 0.02M phosphate buffer, 0.25 M sodium chloride, 0.1% sodium azide, pH 7.6.
Format: Purified

储存及稳定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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