Anti-MSL-2 Antibody, clone 10C3.1

产品说明

一般描述

E3 ubiquitin-protein ligase MSL2 (UniProt Q9HCI7; also known as Male-specific lethal-2 homolog, Male-specific lethal-2 homolog 1, Male-specific lethal 2-like 1, MSL-2, MSL2-like 1, RING finger protein 184) is encoded by the MSL2 (also known as KIAA1585, MSL2L1, RNF184) gene (Gene ID 55167) in human. MSL2 (male-specific lethal 2) was originally identified in Drosophila and characterized as a gene whose mution causes male-specific lethality. Human MSL (hMSL) orthologues are found in an evolutionary conserved complex known as the hMOF (Males absent on the first, human) complex that is composed of hMOF, hMSL1, hMSL2, hMSL3, and Nucleoprotein 153 (NUP153). MSL2 is a RING domain-containing E3 ligase that targets the tumor suppressor p53 Lys351 for monoubiquitination, exposing a nuclear export motif within p53 as well as releasing p53 from MDM2. MSL2 overexpression causes an upregulation of mitochondrial-dependent apoptosis as a result of p53 cytoplasmic accumulation. hMSL1/hMSL2 heterodimer is reported to ubiquitinate histone H2B on Lys34 (H2BK34ub), which promotes transcription activation by stimulating the activities of MLL/SET1 family H3K4 methyltransferases and the H3K79 methyltransferase DOT1L. In addition, both hMSL1 and hMOF are reported to be modified by hMSL2 following DNA damage induction, and hMSL2-depletion causes impaired non-homologous end joining (NHEJ) repair in human cells.

特异性

Expected to react with both spliced isoforms of human MSL2 reported by UniProt (Q9HCI7).

免疫原

Epitope: Internal (C-terminal half).
GST-tagged recombinant human MSL-2 C-terminal fragment.

应用

Anti-MSL-2 Antibody, clone 10C3.1 is an antibody against MSL-2 for use in Western Blotting, Immunocytochemistry.
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of HepG2 and HEK293 cell lysates.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MSL-2 in HUVECs, A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of LNCaP cell lysate.

目标描述

~62 kDa observed. 62.54 kDa (isoform 1) and 54.34 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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