Anti-mPTGES-1

产品说明

一般描述

Prostaglandin E synthase (UniProt: O14684; also known as EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein) is encoded by the PTGES gene (Gene ID: 9536) in human. mPGES -1 is a multi-pass membrane protein that is integral membrane, inducible and glutathione-dependent terminal synthase in the prostanoid biosynthesis pathway and acts downstream cyclooxygenases (COX) catalyzing the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Functionally, mPGES-1 is coupled to COX-2 and the induction of these two enzymes by pro-inflammatory cytokines, growth factors or endotoxin leads to a surge of PGE2 synthesis by inflammatory cells. It is strongly expressed with COX-2 in synovial fibroblasts and macrophages. In cases of osteoarthritis, mPGES-1 and COX-2 are strongly expressed in the chondrocytes and the synovial lining cells. mPGES-1 also play a role in the pathogenesis of ischemic stroke and many neurodegenerative diseases. In Alzheimer disease brains higher expression of both COX-2 and mPGES-1 is observed. Higher expression of mPGES has also been reported in several cancers and this higher expression has been correlated with vascular invasion and worse prognosis in colorectal cancer. A549 and DU145 xenografts lacking mPGES-1 display significantly reduced growth rates. (Ref.: Korotkova, M., and Jakobsson, PJ (2014). Basic Clin. Pharmacol. Toxicol. 114, 64-69).

特异性

This polyclonal antibody specifically detects mPGES-1 in human.

免疫原

His-tagged full-length recombinant human microsomal prostaglandin E synthase.

应用

Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected mPTGES-1 in human tonsil and human placenta tissue.

Western Blotting Analysis: A representative lot detected mPTGES-1 in human chondrocytes (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9) and wild-type mixed glial cultures (Straccia, M., et. al. (2013). Glia. 61(10):1607-19).

Immunofluorescence Analysis: A representative lot detected mPTGES-1 in Wild-type mixed glial cultures (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

Western Blotting Analysis: A representative lot detected mPTGES-1 in PNE inhibitedmPGES-1proteinexpressioninIL-1 -stimulated SK-N-SH cells (Olajide, O.A., et. al. (2014). J Ethnopharmacol. 152(2):377-83).

Immunohistochemistry Analysis: A representative lot detected mPTGES-1 in fresh frozen brain tissue (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

Detect Prostaglandin E synthase using this rabbit polyclonal Anti-mPTGES-1, Cat. No. ABS2192 that has been tested in Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence, and Western Blotting.

质量

Evaluated by Western Blotting in recombinant microsomal Prostaglandin E synthase 1 protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mPTGES-1 in recombinant microsomal Prostaglandin E synthase 1 protein.

目标描述

~17 kDa observed; 17.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Unpurified
Rabbit polyclonal antiserum without azide.

Unpurified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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