Anti-monoubiquitin H2A Antibody (Lys119)

产品说明

一般描述

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The ubiquitination of Histones plays essential roles in the regulation of DNA related processes.

免疫原

Branch peptide corresponding to human monoubiquitin H2A (Lys119).

应用

Western Blotting Analysis: A representative lot from an independent laboratory detected monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Detect Histone H2A using this rabbit polyclonal antibody, Anti-monoubiquitin H2A Antibody (Lys119) validated for use in western blotting, ChIP, IP and ICC.

质量

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.

目标描述

~23 kDa observed. Uniprot describes one isoform at 14 kDa; however, this protein may be observed at ~23 kDa due to monoubiquitination.

外形

Purified rabbit polyclonal in buffer containing 90 mM Tris-glycine pH8, 50 mM Nacl, and 10% glycerol with 0.05% sodium azide
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
HeLa acid extract

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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