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Anti-monoglycylated Tubulin Antibody, clone TAP 952

品牌
Sigma-Aldrich
货号
MABS277
规格纯度
clone TAP 952, from mouse
参考价格
4345.35 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments in Paramecium indicates that the polyglycylation level of tubulin is highly regulated at the cell level. In Paramecium, the TAP 952 antibody is reactive on cytoplasmic and axonemal tubulin. In metazoan cells and/or cell lines, it is specific for tubulin of motile cilia and primary cilia, and thus useful for motile cilia and primary cilia detection.

特异性

This antibody recognizes the C-terminus of monoglycylated alpha and beta-tubulins.
Can be used as a marker for motile cilia.

免疫原

Electroeluted Paramecium axonemal tubulin
Epitope: Amino acids 427-449 of monoglycylated alpha-tubulin and 427-442 of monoglycylated beta-tubulin (Bré et al., 1998, Mol. Biol. Cell 9, 2655-2665)

应用

Research Sub Category
General Post-translation Modification
Immunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Detect Tubulin using this mouse monoclonal antibody, Anti-monoglycylated Tubulin Antibody, clone TAP 952 validated for use in western blotting, Immunofluorescence & Dot Blot.
Research Category
Signaling

质量

Evaluated by Western Blotting on Paramecium total cytoskeletal extract.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.

目标描述

~50 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆TAP 952, monoclonal
species reactivityporcine, sea urchin, snail, Drosophila, human, mouse, sheep
technique(s)dot blot: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型IgG1κ
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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