Anti-Mineralocorticoid Receptor Antibody, clone 6G1

产品说明

一般描述

Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.

特异性

Epitope is present in all 3 alternative spliced isoforms.

免疫原

Conjugated linear peptide corresponding to rat Mineralocorticoid Receptor.

应用

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in various rat tissues including kidney, hippocampus, choroid plexus, cerebellum, colon, heart, and coronary artery (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).

质量

Evaluated by Western Blotting in M1MR cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.

目标描述

~107 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息