产品说明
一般描述
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
特异性
Recognizes Mi-2β (CHD-4)
免疫原
Recombinant protein corresponding to Human Mi-2β (CHD-4).
应用
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.
ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.
Research Sub Category
Chromatin Biology
Histone Modifying Proteins
This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
质量
Evaluated by Western Blotting in a Jurkat cell lystate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.
目标描述
~ 220 kDa observed
外形
Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 17H11, monoclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human |
| technique(s) | ChIP: suitable (ChIP-seq) western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| NCBI登记号 | NP_001264 |
| UniProt登记号 | Q14839 |
| 运输 | wet ice |
| Gene Information | human ... CHD4(1108) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
