位置:首页 > 品牌 > Sigma-Aldrich >

Anti-methyl-CARM1 (Arg551) Antibody

品牌
Sigma-Aldrich
货号
ABE430
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

CARM1, also known as Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4, and encoded by the gene name CARM1 and PRMT4, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. CARM1 methylates (mono- and asymmetric dimethylation) the the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Upon gene activation CARM1 together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ′Arg-17′ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. CARM1 is overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

免疫原

Linear peptide corresponding to human CARM1 (Arg551) methylated at R551.
Epitope: Methylated R551

应用

Research Sub Category
Nuclear Receptors
This Anti-methyl-CARM1 (Arg551) | ABE430 antibody is validated for use in WB, IP for the detection of methyl-CARM1 (Arg551) | ABE430.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: A representative lot immunoprecipitated methyl-CARM1 (Arg551) in vitro methylation of a control buffer, 15 mg nuclear lysates, and 15 mg cytoplasmic lysates from CARM1/MEF cells by 1 mg CARM1 WT and CARM1 R551K (Kuhn, P., et al. (2011). Nucleic Acids Research. 39(7):2717-2726).

质量

Evaluated by Western Blotting in HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected methyl-CARM1 (Arg551) in 10 µg of HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.

目标描述

~62 kDa observed

外形

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 10% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NP_954592
UniProt登记号Q86X55
运输dry ice
Gene Informationhuman ... CARM1(10498)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号