产品说明
一般描述
Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.
特异性
Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein. Does not cross-react with TCF-1 or TCF-4.
免疫原
Recombinant full length LEF-1 protein.
Epitope: all isoforms
应用
Research Sub Category
Neural Stem Cells
Hematopoietic Stem Cells
Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10 is an antibody against LEF-1 for use in WB.
Western blot
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
联系
Replaces: 04-1130
外形
Format: Purified
Purified immunoglobulin. Provided as solution in phosphate buffered saline with 0.08% sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
分析说明
Control
Jurkat cell lysate and human colon adenocarcinoma tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1C3.1D10, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| input | sample type hematopoietic stem cell(s) |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_016269.2 |
| UniProt登记号 | Q9UJU2 |
| 运输 | dry ice |
| Gene Information | human ... LEF1(51176) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
