产品说明
一般描述
Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis.
特异性
Reacts with the 80 kDa fragment of the M-chain of human merosin.
Cross reacts with monkey and rabbit merosin.
免疫原
Purified human merosin
应用
Research Category
Cell Structure
Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.
ELISA:
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.
Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.
Affinity chromatography:
A previous lot of this antibody was used in IAP.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Research Sub Category
ECM Proteins
质量
Routinely evaluated by Western Blot on Human Placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
目标描述
80 kDa
外形
Unpurified ascites in buffer containing no preservatives.
Unpurified
储存及稳定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
分析说明
Control
Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 5H2, monoclonal |
species reactivity | human, monkey, rabbit |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable cell culture | mammalian: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_000426.3 |
UniProt登记号 | P24043 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |