Anti-Lamin A and C Antibody, clone 2F4.1

产品说明

一般描述

Lamin A/C (UniProt: P48679; also known as LMNA) is encoded by the Lmna (also known as Lmn1) gene in rat. Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).

特异性

Clone 2F4.1 detects Lamin A/C in human and rat tissues. It targets an epitope within 148 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 148 amino acids from the internal region of rat Lamin A/C.

应用

Research Category
Cell Structure
Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Lamin A and C in rat cerebral cortex, rat hippocampus, rat cerebellum, and human liver tissue sections.

Anti-Lamin A and C, clone 2F4.1, Cat. No. MABT545,is a mouse monoclonal antibody that detects Lamin A/C and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in WI-38 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lamin A and C in WI-38 cell lysate.

目标描述

~74/65 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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