产品说明
特异性
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.
免疫原
Human epidermal cells
应用
Research Category
Cell Structure
Anti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
Immunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Research Sub Category
Cytokeratins
联系
Replaces: 04-588
外形
Format: Purified
Liquid in PBS. Contains 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| 克隆 | AE3, monoclonal |
| species reactivity | rabbit, bovine, monkey, mouse, human, rat, chicken |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_002273.2 |
| UniProt登记号 | P05787 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
