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Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 647 Conjugate

品牌
Sigma-Aldrich
货号
MAB2079-AF647
规格纯度
clone HUTS-4, from mouse, ALEXA FLUOR® 647
参考价格
6358.33 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interacts with various integrin alpha subunits (including alpha 1, 2, 3, 4, 5 and 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

特异性

Monoclonal antibody HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as HUTS-4 which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody HUTS-4 recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

免疫原

Epitope: A.a. 355-425 region of the common β1 subunit of VLA integrins.
Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.

应用

Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in HeLa and A431 cells.
The unconjugated antibody (Cat. No. MAB2079Z) is shown to be suitable also for Western blotting, ELISA, functional assay, and immunohistochemistry.

This Anti-Integrin β1, activated, clone HUTS-4, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of Integrin β1.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin β1 in NIH/3T3 cells.

目标描述

~88 kDa observed

外形

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
偶联物ALEXA FLUOR® 647
抗体形式purified antibody
antibody product typeprimary antibodies
克隆HUTS-4, monoclonal
species reactivitymouse, human
species reactivity (predicted by homology)rat (based on 100% sequence homology)
technique(s)immunocytochemistry: suitable
同位素/亚型IgG2a
NCBI登记号NM_002292.3
UniProt登记号P05556
运输wet ice
Gene Informationhuman ... ITGB1(3688)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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