产品说明

一般描述

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

特异性

Reacts with the human integrin alphaVbeta5.

免疫原

Human UCLA P3 cells (lung carcinoma)

应用

Research Category
Cell Structure
Research Sub Category
Integrins
Inhibition of attachment of HT29 cells to vitronectin: >1:1,000

Inhibition of attachment of carcinoma and melanoma cells to vitronectin.

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)

Immunocytochemistry: 1:1500. For use on human carcinomas, normal epithelium and keratinocytes (Acetone Fixed Only).

Immunoprecipitation

FACS

EIA

Optimal working dilutions must be determined by the end user.
This Anti-Integrin αVβ5 Antibody, clone P1F6 is validated for use in FC, IC, IH, IP, FUNC for the detection of Integrin αVβ5.

外形

Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Epithelium, keratinocytes

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息