产品说明
特异性
Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.
应用
Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL.
Optimal working dilutions must be determined by end user.
Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6.
外形
Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide.
Format: Purified
储存及稳定性
Store at +2-8°C for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | rat |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | MA6, monoclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgG2κ |
| 适用性 | not suitable for Western blot |
| NCBI登记号 | NM_000210.2 NM_001079818.1 |
| UniProt登记号 | P23229 |
| 运输 | wet ice |
| Gene Information | human ... ITGA6(3655) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
