产品说明

特异性

Reacts with the collagen receptor VLA-2 (alpha-2beta-1) integrin complex.

应用

Research Category
Cell Structure
Research Sub Category
Integrins
flow cytometry: 10 μg/mL

Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1

immunohistochemistry in frozen tissue sections: 5 μg/mL

Immunohistochemistry in paraffin sections: 1:50. Block endogenous peroxidase using 0.5% H202 in 80% ethanol, 30 min and unspecific reactions using NGS 1:5 in PBS, 20min RT. Bouin′s fixative solution has been used successfully. Incubation time, conditions: 24 h; 4°C; pretreatment: 0.1% trypsin, 30 min, 37°C. Intracytoplasmic staining near cell membranes.

Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.

Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.

Final working dilutions must be determined by end user.
Anti-Integrin α2β1 Antibody, clone BHA2.1 is an antibody against Integrin α2β1 for use in FC, IH, IH(P) & IP.

外形

Protein A purified
Purified immunoglobulin from Protein A chromatography. Presented in liquid in 0.02M PB pH 7.6, 0.25M NaCl, 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Skin, CD4 & CD8 positive T cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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