产品说明
一般描述
Anti-Human IgG2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the HP-6014 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG2 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively.
免疫原
Purified human IgG2 myeloma protein
应用
Anti-Human IgG2 antibody, Mouse monoclonal may be used in immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunofluorescence, hemagglutination (HA) and hemagglutination inhibition.
生化/生理作用
Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL) and an IgG1 response in the spleen. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes while IgG2 and IgG4 autoantibodies are much less efficient. The amount of the different IgG subclasses present in the blood shows variation with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is found in patients of ataxia telangiectasia. Low IgG2 levels were found in patients with Systemic lupus erythematosus (SLE) and juvenile diabetes mellitus.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
基本信息
NACRES | NA.46 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | HP-6014, monoclonal |
形式 | buffered aqueous solution |
species reactivity | human |
浓度 | ~1.0 mg/mL |
technique(s) | immunofluorescence: suitable indirect ELISA: 0.07-0.15 μg/mL using 1 μg/mL Human IgG2 myeloma for coating |
同位素/亚型 | IgG1 |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |