产品说明
一般描述
There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the ′beads on a string′ structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.
特异性
Due to the highly conserved nature of histones, this antibody is predicted to have wide species reactivity.
The antibody binds specifically to an antigen determinant, which is present on all five histone proteins. The antibody recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity.
免疫原
Purified nuclear fractions of HeLa cells
应用
Detect Histone with Anti-Histone Antibody, clone H11-4 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB.
Research Sub Category
Histones
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Immunocytochemistry:
A 1-5 µg/mL concentration of a previous lot was used on Immunocytochemistry.
Immunohistochemistry:
A 1-5 μg/mL concentration of a previous lot was used on Immunohistochemistry.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
质量
Routinely evaluated by Western Blot on Jurkat lysates.
外形
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, pH 7.6 with 0.25M NaCl and 0.1% sodium azide
Protein A purified
储存及稳定性
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided.
分析说明
Control
All tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | H11-4, monoclonal |
species reactivity | rat, bovine, human, hamster, Xenopus, opossum |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |