产品说明
一般描述
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
特异性
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
免疫原
Recombinant mouse HP1alpha.
应用
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
目标描述
25 kDa
外形
Liquid ascites fluid containing no preservative.
Unpurified
分析说明
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | 2HP-2G9, monoclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_012117.1 |
| UniProt登记号 | P45973 |
| 运输 | wet ice |
| Gene Information | human ... CBX5(23468) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
