产品说明
一般描述
GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.
Monoclonal Anti-GSDMD recognizes human GSDMD.
Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.
免疫原
Synthetic peptide of human GSDMD, conjugated to KLH
应用
The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.
生化/生理作用
Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.
外形
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
其他说明
This product is for R&D use only, not for drug, household, or other uses.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | GSD-14, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~53 kDa |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 2-4 μg/mL using human Jurkat cell extract immunofluorescence: 5-10 μg/mL using human A431 cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P57764 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... GSDMD(79792) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
