Anti-Glutamine Synthetase Antibody, clone GS-6

产品说明

一般描述

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

特异性

Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.

免疫原

Glutamine synthetase purified from sheep brain.

应用

Research Sub Category
Neurofilament & Neuron Metabolism
Western blot: 1:1,000-1:5,000

Immunohistochemistry on rat brain

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Detect Glutamine Synthetase using this Anti-Glutamine Synthetase Antibody, clone GS-6 validated for use in ELISA, IH, IH(P) & WB with more than 45 product citations.

目标描述

45 kDa

外形

Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.

储存及稳定性

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

分析说明

Control
Rat brain tissue, rat brain cytosolic fraction extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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