产品说明
一般描述
Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma).
免疫原
Human plasma gelsolin
应用
Anti-Gelsolin antibody, Mouse Monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
生化/生理作用
Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | GS-2C4, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~85 kDa |
| species reactivity | monkey, canine, human, mouse, rabbit |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 1-2.5 μg/mL using MDCK cell line extract immunofluorescence: suitable immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P06396 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... GSN(2934) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
