Anti-FucT-III Antibody

产品说明

一般描述

Alpha-(1,3)-fucosyltransferase 3 (FucT-III; Galactoside 3(4)-L-fucosyltransferase) is highly expressed in the small intestine, colon, stomach, and kidney. It is a member of the glycosyltransferase family of proteins which are involved in a glycosylation pathway that attaches fucose to Lewis epitopes of target molecules. This type of post-translational modification allows modified (fucosylated) molecules to recognize specific target substrates. Fucosyltransferases may therefore play an important role in communication at the cell surface. FucT-III may play a role in blood group determination as it is involved in the expression of antigens such as Lewis B, sialy Lewis X, Vim-2, and Lewis A.

特异性

This antibody recognizes FucT-III at the lumenal region.
No homology to Rat and Mouse was found.

免疫原

Epitope: Lumenal region
KLH-conjugated linear peptide corresponding to the lumenal region of human FucT-III.

应用

Research Category
Cell Structure
Research Sub Category
Glycobiology
Anti-FucT-III Antibody detects level of FucT-III & has been published & validated for use in Western Blotting.
Western Blot Analysis: 1 µg/mL from a representative lot detected FucT-III on 10 µg of transfected lysate and did not detect the non transfected lysate.

质量

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected FucT-III on 10 µg of human liver tissue lysate.

目标描述

~50 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~50 kDa due to high glycosylation. (Christensen, L, et al. (2000). Glycobiology. 10(9):931-939.)

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human Liver Tissue Lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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