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Anti-Fox-3 Antibody

品牌
Sigma-Aldrich
货号
ABN51
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5426.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.

特异性

This antibody recognizes Fox-3 at the N-terminus.

免疫原

GST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.
Epitope: N-terminus

应用

Western Blotting Analysis: 2 µg/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

质量

Evaluated by Western Blot in mouse e16 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.

目标描述

~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse e16 brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, rat, human
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号NP_001034256
UniProt登记号Q8BIF2
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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