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Anti-Filamin A Antibody, clone TI10

品牌
Sigma-Aldrich
货号
MAB1680
规格纯度
ascites fluid, clone TI10, Chemicon®
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.

特异性

Human filamin (actin-bidning protein). Recognizes unprocessed (270-280 kDa) and the C-terminal 90 kDa calpain cleavage fragment of filamin (Aakhus, 1992).

免疫原

Human platelet protein

应用

Research Category
Cell Structure
Detect Filamin A using this Anti-Filamin A Antibody, clone TI10 validated for use in IP, WB, IH, IH(P).
Research Sub Category
Cytoskeleton
Immunoblotting: 1:250 to 1:1000

Immunoprecipitation:Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300μL of cell lysate (3-5mg/ml total protein is suggested). Incubation is 4 hours RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kDa fragement visualization.

Immunofluorescence: 1:50 to 1:200 using standard ABC technique. Suitable for staining of paraffin embedded sections (lower dilutions). High temperature citrate buffer antigen retrieval technique recommended.

Optimal working dilutions must be determined by end user.

联系

Replaces: CBL229

外形

Ascites. Liquid

储存及稳定性

Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

分析说明

Control
Positive control tisse: skin.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆TI10, monoclonal
species reactivitybovine, human
should not react with mouse, rat
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1
NCBI登记号NM_001110556.1
NM_001456.3
UniProt登记号P21333
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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