产品说明
一般描述
ETS family members play an important role in mammalian development and have been implicated in the regulation of gene expression during a variety of biologic processes including cell growth and differentiation. Defects or deficiencies, as well as overexpression of ETS family members can have major developmental effects as well as aid in tumor development. ETS translocation variant 5 protein (ETV5) also known as ETS-related protein ERM is a nuclear localized protein that binds to DNA sequences containing the consensus nucleotide core sequence of GGAA and is a member of the very large ETS transcription factor family. Early experiments in worms suggested that ETV5 might play a role in the development of the dopaminergic nervous system; however that has not proved to be the case in mammals. However, more recent research has indicated ETV5 playing an important role in the development of reproduction and in the development of ovarian cancer. ETV5 controls the expression of critical prostaglandins needed for ovarian development and ETV5 deficient mice show complex infertility phenotypes both in males and females. In males, the infertility phenotype exhibited by ETV5 deficiency is sexually dimorphic, and it involves both somatic cells and germ cells. In Etv5-/- female mice, the problem is more complicated by hormonal involvement and results in more a complex phenotype. EMD-Millipore’s Anti-ETV5 mouse monoclonal antibody has been tested in western blot against Jurkat, NIH3T3 and MCF-7 cell lysates.
免疫原
Purified recombinant fragment of human ETV5 expressed in E. Coli.
应用
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-ETV5 Antibody, clone 3H3 is a highly specific mouse monoclonal antibody & that targets Ets & has been tested in western blotting.
质量
Evaluated by Western Blotting in Jurkat, NIH/3T3 and MCF-7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV5 in Jurkat, NIH/3T3 and MCF-7 cell lysates.
Optimal working dilutions must be determined by end user.
目标描述
~58 kDa observed. Uncharacterized bands may appear in some lysate(s).
外形
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Jurkat, NIH/3T3 and MCF-7 cell lysates
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 3H3, monoclonal |
species reactivity | human |
technique(s) | western blot: suitable |
同位素/亚型 | IgG1 |
UniProt登记号 | P41161 |
运输 | wet ice |
Gene Information | human ... ETV5(2119) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |