产品说明
一般描述
Epithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.
特异性
This antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.
免疫原
HES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)
Epitope: Unknown
应用
This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).
Flow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.
Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
Research Sub Category
Pluripotent & Early Differentiation
Adhesion (CAMs)
Research Category
Stem Cell Research
质量
Evaluated by Immunocytochemistry in human embryonic stem cells.
Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.
目标描述
~ 40 kDa
外形
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human embryonic stem cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
克隆 | mab 8, monoclonal |
species reactivity | human |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_002345.2 |
UniProt登记号 | P16422 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |