产品说明
一般描述
Anti-EpCAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma Ber-EP4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human breast carcinoma cell line MCF-7. EpCAM (Epithelial cell adhesion molecule) is a highly conserved type I transmembrane glycoprotein. EpCAM is present on most epithelia tissues of the adult body and in undifferentiated rather than differentiated embryonic stem cells.
免疫原
human breast carcinoma cell line MCF-7
应用
Anti-EpCAM antibody has been used in:
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
生化/生理作用
EpCAM (Epithelial cell adhesion molecule) regulates cell-cell contact adhesion strength and tissue plasticity and epithelial cell proliferation and differentiation. Mutations in EpCAM are associated with several intestinal abnormalities such as Lynch syndrome, and congenital tufting enteropathy (CTE). Anti-EpCAM has been proved valuable for the distinction of undifferentiated primary or metastatic tumors from non-epithelial tumors, bile duct cells from hepatocytes in certain liver diseases, and between epithelial and normal reactive or neoplastic mesothelial cells from carcinoma cells.
外形
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | Ber-EP4, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| technique(s) | immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line. immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections. immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P16422 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
