产品说明
一般描述
ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) is a putative mannose-binding lectin. EDEM1 is localized to the endoplasmic reticulum (ER) mainly as a soluble glycoprotein.
免疫原
synthetic peptide corresponding to an internal region within human EDEM1, conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse and by 2 amino acids in rat.
应用
Anti-EDEM1(internal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
生化/生理作用
Over-expression of ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) leads to endoplasmic reticulum (ER)-associated degradation (ERAD) by stimulating the release of terminally misfolded glycoproteins from calnexin. EDEM degrades misfolded glycoproteins in an N-glycan dependent manner. Three EDEM homologues, EDEM1, EDEM2 and EDEM3 are transcriptionally upregulated by activated inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1 (XBP1) branch. It interacts with the COOH-terminus of calnexin and lacks mannosidase activity.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 75/65 kDa |
| species reactivity | mouse, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells over expressing mouse EDEM1. western blot: 2-4 μg/mL using whole extracts of human Jurkat cells. |
| UniProt登记号 | Q92611 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... EDEM1(9695) mouse ... Edem1(192193) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
