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Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody

品牌
Sigma-Aldrich
货号
MABE977
规格纯度
clone BF18.11, from mouse
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.

免疫原

His-tagged recombinant protein corresponding to mouse E3 ubiquitin-protein ligase UHRF1.

应用

Research Sub Category
Nuclear Receptors
This Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of E3 ubiquitin-protein ligase UHRF1.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in 5 mg of NIH/3T3 cell lysate (Roberto Papait, FIRC Institute of Molecolar Oncology).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of NIH-3T3 cell lysate.

目标描述

~88 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆BF18.11, monoclonal
species reactivitymouse
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001104548
UniProt登记号Q8VDF2
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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