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Anti-Dopamine D2 Receptor Antibody

品牌
Sigma-Aldrich
货号
AB1558
规格纯度
serum, Chemicon®
参考价格
5529.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Recognizes Dopamine D2 Receptor (D2R). Recognizes both native and denatured forms of D2R (110, 68 and 47 kDa bands by Western blot using rat striatal positive control (Catalog Number AG282)). AB1558 inhibits binding of [3H]YM-09152-2, a known D2R antagonist and (125)I-NAPS, a photoligand. It inhibits binding of [3H]YM-09152-2 and PPHT-fluorescein (an agonist of D2R) and reacts with 110 kDa D2R band in fibroblasts transfected with rat D2R cDNA. AG284 is available for blocking experiments; AG284 is not recommended as a positive control for western blots.

免疫原

Amino-terminal peptide sequence (aa 24-34) near or at the ligand binding domain of rat D2R (1,2).

The immunogen shows 100% homology with mouse, 90% with human and bovine and 72% with canine D2R receptors. An antibody made to the same sequence has shown reactivity with human, lobster, squid and crawfish.

应用



Immunohistochemistry: 4% PFA fixed, frozen sections, 1:200, 24 hour incubation at room temperature, DAB detection. {Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105.

Immunocytochemistry: 4% PFA fixed cells 10-15′, permeabilized with 0.2-0.3% triton X-100, 1:100, o/N 4C for fluorescence {Sun, W et al (2005) Stem Cells 23(7):931-945, http://stemcells.alphamedpress.org/cgi/content/full/23/7/931}.

Western blot: 1:1,000-1:5,000 (Chemiluminescence technique) Higher concentration of antibody may be necessary when using low concentration samples or when using milk in antibody diluents. It may be necessary to load 100-200 μg of purified striatal or other tissue membranes to visualize the DR2 bands. The antibody has been shown to recognize both native and denatured DR2 bands.

ELISA: 1:10,000-1:50,000 [1 μg/mL D2R peptide (Cat. Number AG284)/well]

Optimal working dilutions must be determined by end user.
Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

目标描述

110, 68 and 47 kDa

外形

Unpurified
Rabbit antiSerum. Liquid containing 0.05% sodium azide.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Brain

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityrat
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_000795.2
NM_016574.2
UniProt登记号P14416
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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