产品说明
一般描述
Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.
免疫原
human p125
应用
Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- co-immunoprecipitation
生化/生理作用
The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.
外形
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
产品性质
质量水平 | 200 |
生物来源 | rat |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | PDG 5G1, monoclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~125 kDa |
species reactivity | human, mouse, rat |
包装 | antibody small pack of 25 μL |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: 0.5-1.0 μg/mL using Jurkat cells extract |
UniProt登记号 | P28340 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... POLD1(5424) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |