产品说明
一般描述
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
特异性
This antibody recognizes the N-terminus of asymmetric Histone H4 dimethylated at Arg17.
免疫原
KLH-conjugated linear peptide corresponding to the N-terminus of human asymmetric Histone H4 dimethylated at Arg17.
应用
Dot Blot (Specificity) Analysis: A 1:200 dilution from a representative lot detected asymmetric Histone H4 dimethylated at Arg17, but not unmethylated Histone H4 or other peptides corresponding to modified histones.
Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H4 dimethylated at Arg17 in MEF-1 cells.
Alexa Fluor® is a registered trademark of Life Technologies
Detect Histone H4 using this rabbit polyclonal antibody, Anti-dimethyl Histone H4 Antibody (Arg17) validated for use in western blotting, Dot Blot & ICC.
质量
Evaluated by Western Blotting in untreated and CARM1 treated Histone H4 recombinant proteins.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected asymmetric Histone H4 dimethylated at Arg17 in CARM1 treated Histone H4 recombinant proteins.
目标描述
~13 kDa observed
外形
Affinity purified
分析说明
Control
Untreated and CARM1 treated Histone H4 recombinant proteins.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | dot blot: suitable immunocytochemistry: suitable western blot: suitable |
| NCBI登记号 | NP_001029249 |
| UniProt登记号 | P62805 |
| 运输 | wet ice |
| Gene Information | human ... HIST2H4B(554313) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
